Identification and characterization of genes encoding phosphoinositide-specific phospholipase C revealed role in drought stress condition in cassava (Manihot esculenta)
DOI:
https://doi.org/10.56764/hpu2.jos.2023.2.3.42-50Abstract
Phosphoinositide-specific phospholipase C (PI-PLC) has been known as one of the key enzymes that involved in the phospholipid hydrolysis. However, the PI-PLC family in cassava has not been fully recorded. In this study, we reported a comprehensive analysis of the PI-PLC family in cassava assembly based on various bioinformatics tools. Particularly, a total of seven members of the PI-PLC family has been identified and annotated in the cassava genome. By using the full-length protein sequence of each member of the PI-PLC family in cassava, we analyzed the properties of these proteins, including the length, size, iso-electric point, instability index, aliphatic index and grand average of hydropathy. Of our interest, we investigated the expression patterns of genes encoding the PI-PLC family in various major organs/tissues in different conditions. Taken together, our study could provide a solid foundation for the PI-PLC family in cassava for further functional characterization towards the improvements of drought stress tolerance in cassava plants.
References
[1] Guira, F., et al., Origins, "Production, and utilization of cassava in Burkina Faso, a contribution of a neglected crop to household food security," Food Sci Nutr 5, pp.415-423, doi: 10.1002/fsn3.408
[2] Olsen, K.M., et al., "Evidence on the origin of cassava: Phylogeography of Manihot esculenta," Proceedings of the National Academy of Sciences 96, pp.5586-5591, doi: 10.1073/pnas.96.10.5586
[3] Hillocks, R.J., et al., "Cassava : biology, production and utilization. 2002," Wallingford, UK; New York: CABI Pub, doi: 10.1079/9780851995243.0000
[4] De Souza, A.P., et al., "Rooting for cassava: insights into photosynthesis and associated physiology as a route to improve yield potential," New Phytol 213, pp.50-65, doi: 10.1111/nph.14250
[5] Chavarriaga-Aguirre, P., et al., "The potential of using biotechnology to improve cassava: a review," In Vitro Cell Dev Biol Plant 52, pp.461-478, doi: 10.1007/s11627-016-9776-3
[6] Nilusha, R.A.T., et al., "Proximate composition, physicochemical, functional, and antioxidant properties of flours from selected cassava (Manihot esculenta Crantz) varieties," Int J Food Sci 2021 6064545 , doi: 10.1155/2021/6064545
[7] Morgan, N.K., et al., "Cassava: Nutrient composition and nutritive value in poultry diets," Anim Nutr 2 253-261, doi: 10.1016/j.aninu.2016.08.010
[8] Shan, Z., et al., "Physiological and proteomic analysis on long-term drought resistance of cassava (Manihot esculenta Crantz)," Scientific Reports 8 17982, doi: 10.1038/s41598-018-35711-x
[9] Nakamura, Y., "Plant Phospholipid Diversity: Emerging Functions in Metabolism and Protein-Lipid Interactions," Trends Plant Sci 22, pp.1027-1040, doi: 10.1016/j.tplants.2017.09.002
[10] Colin, L.A., et al., "Phospholipids across scales: lipid patterns and plant development," Curr Opin Plant Biol 53 1-9, doi: 10.1016/j.pbi.2019.08.007
[11] Ali, U., et al., "The functions of phospholipases and their hydrolysis products in plant growth, development and stress responses," Prog Lipid Res 86, pp.101-158, doi: 10.1016/j.plipres.2022.101158
[12] Fang, Y., et al., "Phospholipase C: Diverse functions in plant biotic stress resistance and fungal pathogenicity," 24, pp.1192-1202, doi: 10.1111/mpp.13343
[13] Rupwate, S.D., et al., "Plant phosphoinositide-specific phospholipase C: an insight," Plant Signal Behav 7 1281-3, doi: 10.4161/psb.21436
[14] Gao, K., et al., "Arabidopsis thaliana phosphoinositide-specific phospholipase C isoform 3 (AtPLC3) and AtPLC9 have an additive effect on thermotolerance," Plant Cell Physiol 55, pp.1873-83, doi: 10.1093/pcp/pcu116
[15] Singh, A., et al., "Comprehensive genomic analysis and expression profiling of phospholipase C gene family during abiotic stresses and development in rice," PLoS One 8 e62494, doi: 10.1371/journal.pone.0062494
[16] Song, F., et al., "Molecular cloning and characterization of a rice phosphoinositide-specific phospholipase C gene, OsPI-PLC1, that is activated in systemic acquired resistance," Physiological and Molecular Plant Pathology 61, pp.31-40, doi: 10.1016/S0885-5765(02)90414-5
[17] Wang, F., et al., "Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max)," PLoS One 10 e0138467,
doi: 10.1371/journal.pone.0138467
[18] Apostolakos, P., et al., "The involvement of phospholipases C and D in the asymmetric division of subsidiary cell mother cells of Zea mays," Cell Motil Cytoskeleton 65 863-75, doi: 10.1002/cm.20308
[19] Zhang, B., et al., "Genome-wide identification and characterization of phospholipase C gene family in cotton (Gossypium spp.)," Sci China Life Sci 61, pp.88-99, doi: 10.1007/s11427-017-9053-y
[20] Wang, X., et al., ''Genome-Wide Identification and Expression Profile Analysis of the Phospholipase C Gene Family in Wheat (Triticum aestivum L.)," Plants, 2020. 9, doi: 10.3390/plants9070885
[21] Bredeson, J.V., et al., "Sequencing wild and cultivated cassava and related species reveals extensive interspecific hybridization and genetic diversity," Nat Biotechnol 34, pp.562-570, doi: 10.1038/nbt.3535
[22] Goodstein, D.M., et al., "Phytozome: a comparative platform for green plant genomics," Nucleic Acids Res 40 D1178-86, doi: 10.1093/nar/gkr944
[23] Wilson, M.C., et al., "Gene expression atlas for the food security crop cassava," New Phytol 213, pp.1632-1641, doi: 10.1111/nph.14443
[24] Barrett, T., et al., "NCBI GEO: archive for functional genomics data sets - update," Nucleic Acids Res 41 D991-5, doi: 10.1093/nar/gks1193
[25] Mistry, J., et al., "Pfam: The protein families database in 2021," Nucleic Acids Res 49 D412-D419, h doi: 10.1093/nar/gkaa913
[26] La, H.V., et al., "Insights into the gene and protein structures of the CaSWEET family members in chickpea (Cicer arietinum), and their gene expression patterns in different organs under various stress and abscisic acid treatments," Gene 819, pp.146-210, doi: 10.1016/j.gene.2022.146210
[27] Chu, H.D., et al., "Identification, structural characterization and gene expression analysis of members of the Nuclear Factor-Y family in chickpea (Cicer arietinum L.) under dehydration and abscisic acid treatments," Int J Mol Sci 19, 32-90, doi: 10.3390/ijms19113290
[28] Gasteiger, E., et al., "ExPASy: The proteomics server for in-depth protein knowledge and analysis, " Nucleic Acids Res 31 3784-8, doi: 10.1093/nar/gkg563
[29] Gasteiger, E., et al., "Protein identification and analysis tools on the ExPASy server," in The proteomics protocols handbook. 2005, Springer, pp.571-607, doi: 10.1385/1-59259-890-0:571
[30] Barrett, T., et al., "NCBI GEO: archive for functional genomics data sets--update," Nucleic Acids Res 41 D991-5, doi: 10.1093/nar/gks1193
[31] Chu Duc Ha, L.X.D., Tran Thi Thanh Huyen, Pham Thi Ly Thu, "Evolutionary analysis and expression profiling of the sweet sugar transporter gene family in cassava (Manihot esculenta Crantz)," Tạp chí Khoa học - Đại học Sư phạm Hà Nội.
[32] Chu Duc Ha, P.T.Q., Pham Thi Ly Thu, Nguyen Van Cuong, Le Tien Dung, "Identification of the sweet transporter gene family in cassava (Manihot esculenta Crantz)," Tạp chí Khoa học - Đại học Sư Phạm Hà Nội.
[33] Zhu, Y., et al., "Antioxidant Enzymatic Activity and Its Related Genes Expression in Cassava Leaves at Different Growth Stages Play Key Roles in Sustaining Yield and Drought Tolerance Under Moisture Stress," Journal of Plant Growth Regulation 39, pp.594-607, doi: 10.1007/s00344-019-10003-4
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Copyright (c) 2023 Lan-Huong Do Thi, Hoang-Minh Ta, Duc-Ha Chu
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