Study on isolating, breeding, and cultivation of Xylaria nigripes (Klotzsch) Cooke

Authors

  • Thi-Thuong Ngo Hanoi Pedagogical University 2, Vinh Phuc, Vietnam
  • Xuan-Phong Ong Hanoi Pedagogical University 2, Vinh Phuc, Vietnam
  • Dinh-Viet Nguyen Minh Dai High School, Phu Tho, Vietnam
  • Thi-Hang Doan Nhan Chinh Secondary School, Hanoi, Vietnam
  • Bich-Phuong Vu Dich Vong Secondary School, Hanoi, Vietnam
  • Viet-Hong La Hanoi Pedagogical University 2, Vinh Phuc, Vietnam

DOI:

https://doi.org/10.56764/hpu2.jos.2024.3.1.88-94

Abstract

The Xylaria nigripes fungus is one of the medicinal materials that was used in traditional medicine. Extracts from the mycelium and fruiting bodies of the X. nigripes showed protecting cells, preventing DNA damage, and reducing the programmed death cell rate. In this study, the fruiting bodies of the X. nigripes were collected to isolate, propagate in the liquid medium, and then cultured to induce the fruiting body formation in the rice medium. The results showed that the X. nigripes was successfully isolated on PDAY (Potato Dextrose Agar Yeast Extract) medium, and the mycelium and spore of the X. nigripes had unique characteristics of the species. The biomass of X. nigripes grew well in a liquid nutrient medium containing 20g glucose, 1g MgSO4.7H2O, 2g KH2PO4, and 5g peptone, reaching the best mass after 21 to 28 days. Using an initial cultured liquid of X. nigripes to culture on rice medium, fruiting bodies were formed and developed after 14 days of culture. This result could potentially be applied to the production of X. nigripes biomass via mycelium and fruit bodies for application in medicine.

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Published

26-04-2024

How to Cite

Ngo, T.-T., Ong , X.-P., Nguyen, D.-V., Doan, T.-H., Vu, B.-P., & La , V.-H. (2024). Study on isolating, breeding, and cultivation of Xylaria nigripes (Klotzsch) Cooke. HPU2 Journal of Science: Natural Sciences and Technology, 3(1), 88–94. https://doi.org/10.56764/hpu2.jos.2024.3.1.88-94

Volume and Issue

Vol. 3 No. 1 (2024): HPU2.Nat.Sci.Tech

Section

Natural Sciences and Technology

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Copyright (c) 2024 Thi-Thuong Ngo, Xuan-Phong Ong , Dinh-Viet Nguyen, Thi-Hang Doan, Bich-Phuong Vu, Viet-Hong La

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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